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Image Search Results
Journal: Bio-protocol
Article Title: Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice
doi: 10.21769/BioProtoc.2294
Figure Lengend Snippet: Cells were fixed with 4% paraformaldehyde 14 days after isolation and subsequently stained for CD31 (BD, 1:500) as an endothelial cell specific marker in combination with α-SMA (pericytes and smooth muscle cells, Acris, 1:200, upper row), Iba1 (microglia, Wako Pure Chemical Industries, 1:100, middle row) and GFAP (astrocytes, Millipore, 1:400, lower row). Scale bars represent 50 μm.
Article Snippet: 12 ,
Techniques: Isolation, Staining, Marker
Journal: Bio-protocol
Article Title: Isolation and Cultivation of Primary Brain Endothelial Cells from Adult Mice
doi: 10.21769/BioProtoc.2294
Figure Lengend Snippet:
Article Snippet: 12 ,
Techniques: Concentration Assay
Journal: Molecular Medicine Reports
Article Title: Fasudil, a Rho-kinase inhibitor, prevents intima-media thickening in a partially ligated carotid artery mouse model: Effects of fasudil in flow-induced vascular remodeling
doi: 10.3892/mmr.2015.4409
Figure Lengend Snippet: Flow-induced vascular remodeling is reduced in fasudil-treated mice. Proliferation and migration of vascular smooth muscle cells were identified by immunohistochemistry on paraffin-embedded sections of the carotid arteries from wild-type C57Bl/6J mice. (A) PCNA expression in the LCA of (a) untreated or (b) fasudil-treated arteries (16.1±2.4% vs. 5.2±2.1%; *** P<0.001). Values are presented as the mean ± standard error of the mean; n=8 (number of vessels). (B) Smooth muscle cell α-actin staining of LCA cross-sections from fasudil-treated and untreated mice 14 days and 28 days subsequent to ligation (vascular smooth muscle cells are stained brown; magnification, ×40; scale bar, 20 µm). PCNA, proliferating cell nuclear antigen; LCA, left common carotid artery.
Article Snippet: The common carotid arteries were cut longitudinally and antibodies against cluster of differentiation (CD)45 monoclonal antibody (mAb; 1:50; cat. no. ZM-0213; ZSGB-BIO), CD68 mAb (1:100; cat. no. UM800047; OriGene Technologies, Inc., Rockville, MD, USA), proliferating cellular nuclear antigen (PCNA) mAb (1:50; cat. no. TA301137; Cell Signaling Technology, Inc., Danvers, MA, USA) and
Techniques: Migration, Immunohistochemistry, Expressing, Staining, Ligation